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Nucleic Acid Hybridization Nucleic acid hybridization is a general method in determining the sequence of homologous DNA, it is also used to differentiate order of genes in a haploid set of chromosomes of a particular organism and the size of limitation fragments that contain such sequence. Meaning, it is possible to study the genetic differences between different organisms and individual here on Earth. A nucleic Acid Hybridization analysis uses five laboratory skills the Restriction fragment preparation: where a restriction enzyme is added to a DNA sample, Electrophoresis: separate the restriction fragment from DNA sample, Blotting: single strands are transferred onto special paper or nylon membranes through capillary action, Radioactive†¦show more content†¦Plasmid C has some similarity from plasmid A but the similar sequences are found on the different size in the fragments. The autoradiograph gives us more information than the agarose gel or its own. In Vitro Mutagenesis In vitro mutagenesis a technique used to introduce specific changes into the sequence of a cloned gene. Mutations alter function of protein product. Mutated gene turned to the host cell, is possible to determine function of the missing normal protein by examining what changes occur in the cell. Methods of In Vitro Mutagenesis: 1. After cloning, the double-stranded plasmid is denatured to obtain a single-stranded DNA template that includes the wild-type version of the DNA of interest. 2. A mutagenic primer is allowed to base-pair with the single-stranded template. The primer consists of two regions that are complementary to template regions on either side of a mismatched region – a region where the base sequence of the mutagenic primer is not complementary and will not pair with the base sequence in the template DNA. 3. Addition of DNA polymerase elongates the primer strand to produce a double-stranded plasmid, one strand representing the original genetic information and the other strand representing the new mutant DNA. 4. The vectors (plasmids) are then used to transform bacterial cells, which replicate both strands of the plasmid. When such a transformed cell first divides,Show MoreRelatedWritten Mode- English Language1518 Words   |  7 PagesUrban Environments Written Mode Introduction Communication is a tool with which we exercise our influence on others, bring out changes in our and others’ attitudes, motivate the people around us and establish and maintain relationships with them. Communication forms a major part of our life and is a social activity. This social activity is pursued verbally through speech, reading and writing or non-verbally through body language. The focus of this report will be mainly on written side of communicationRead MoreWritten and Spoken English Language Essay726 Words   |  3 PagesWritten and Spoken English Language The English language consists both of written and spoken features. Until recently, items and structures most typically found in spoken language have not been fully described. Most grammars of English have had a bias towards the written language. They are many differences between spoken and written English. Text A and B show written and spoken versions of an account of a days activities. Text A is a written account of these activities, while Text B is aRead MoreThe Features of Written Language and Speech Essay604 Words   |  3 PagesThe Features of Written Language and Speech In English language there are two different ways of actually presenting language. These are written language and speech. These two factors of speech both include many different features between themselves. These features are mostly opposite to each other as they are completely different ways of presenting language. Written language is structured into paragraphs unlike general speech which is hardly thought about before beingRead More The poem Carrickfergus was written by an Irish writer called Louis700 Words   |  3 PagesThe poem Carrickfergus was written by an Irish writer called Louis MacNeice in The 20th Century. The poem Carrickfergus was written by an Irish writer called Louis MacNeice in The 20th Century. It was written in four line stanzas and now I am going to analyse this poem and talk about each stanza. In the first line of the first stanza, we notice that the child Louis MacNeice is using specific detail in his poem as he says he was born in Belfast between the mountain and the gantries.Read MoreMulti-Modal Essay1030 Words   |  5 Pagesuse such as :) for a smile. The message is then carried on by the next participant replying as â€Å"not really†¦x†. There is a use of an ellipsis, and it could be used to signify the meaning that the message is continuous even though there is nothing written after it. The same participant also states â€Å"dunno tbh but somethings up with my stomach/ribs :/ idk I didnt know ya liked her.......†. She uses initialism to shorten the time to type this message. She also uses contractions as well to shorten theRead MoreThe Process Of Learning For Read Can Be Difficult For Young Children1096 Words   |  5 Pagestheir vocabulary and understanding of words, at a relevant level with the support and guidance of the educator. Many children are fortunate enough to be surrounded by print and written language from a very young age. When exposed to signs, posters, books and even imitation writing, children are developing the concept that written words have a meaning, just like spoken words do (Tompkins, Campbell Green, 2012, p. 104). Parents and teachers alike have enormous opportunities to expose children to conceptsRead More Flannery O’Connor’s Short Story A Good Man is Hard to Find Essay examples1021 Words   |  5 Pagesevery true Southern woman. She related stories of old mansions and of the little ‘pickaninny’ by a door. This was not a racial comment because for it to be there would have to be an intent to insult an African American and there was not. This was written to further convey the notion of her embodying all the true characteristics of Southern women, including their adherence to devout Christianity. The Misfit exemplifies the cold, contemporary world. In the conversation The Misfit’s declares aboutRead MoreThe Necklace Short Story1468 Words   |  6 Pagesâ€Å"The necklace† was written in French and published in a French newspaper in 1884. Maupassant wrote the short story about a young married women, who is a fantasist, certain that she should be living a complete different lifestyle. Regardless of the period it was written, portrays Matilde as being a jealous egotist who lives her life through self-delusion and self-pity which are all timeless and universal themes. The presentation of women is very particular, if you weren’t a rich women you wouldn’tRead MoreThe Mystery Of The Myth925 Words   |  4 Pagesas it was years ago it still has left its mark on history. Many countries like to borrow, Japan is one of those that like to borrow a lot. Japan is a country that like’s to borrow many things and language is one of them. First Japan had their own written language â€Å"hiragana†, but then they decided to completely adapt a new one from the very successful Tang Dynasty. Japan created a new writing system called â€Å"kanji† even though they had their own. In more contemporary time Japanese is still continuingRead MoreThe Importance Of Reading And Writing For Writing893 Words   |  4 Pagesreading and writing every day. In this essay I will explain why I believe that literature is important. I will give personal examples about why I think that reading and writing are important. We have used written language from the beginning of time. Our ancestors from a long time ago left us written records, symbolism, and pictures to tell us a story about their lives. A lot of these languages I personally cannot read but they are just as important as our language is now. If they did not leave these